Nuclear RNA surveillance
Recent studies indicate that transcription is pervasive in eukaryotes, producing a complex network of noncoding RNAs (ncRNAs) that exist stably in cells or are rapidly degraded. To date, however, little is known about the pathways and mechanisms that promote RNA surveillance and quality control.
Our work on the exosome complex of 3’-5’ exonucleases has uncovered a novel mechanism of transcription termination that prevents the accumulation of aberrant read-through RNAs and transcriptional interference at neighboring genes. We also made a major breakthrough into the understanding of how polyadenylation site selection is intimately coordinated with RNA quality control.
Bitton, D. A., S. R. Atkinson, C. Rallis, G. C. Smith, D. A. Ellis, Y. Y. Chen, M. Malecki, S. Codlin, J. F. Lemay, C. Cotobal, F. Bachand, S. Marguerat, J. Mata and J. Bahler (2015). “Widespread exon skipping triggers degradation by nuclear RNA surveillance in fission yeast.” Genome Res 25: 884-896.
Lemay, J. F., M. Larochelle, S. Marguerat, S. Atkinson, J. Bahler and F. Bachand (2014). “The RNA exosome promotes transcription termination of backtracked RNA polymerase II.” Nat Struct Mol Biol 21: 919-926.
Larochelle, M., J. F. Lemay and F. Bachand (2012). “The THO complex cooperates with the nuclear RNA surveillance machinery to control small nucleolar RNA expression.” Nucleic Acids Res 40: 10240-10253.
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Recent studies indicate that transcription is pervasive in eukaryotes, producing a complex network of noncoding RNAs (ncRNAs) that exist stably in cells or are rapidly degraded.